Anti-iNOS antibody
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概述
- 产品描述Nitric oxide (NO) has a broad range of biological activities and has been implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOSs), the enzymes responsible for synthesis of NO, contain an N-terminal oxygenase domain and a C-terminal reductase domain. NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells. The inducible form of NOS, NOS2 (also designated iNOS), is Ca2+-independent and is expressed in a broad range of cell types.
- 产品名称Anti-iNOS antibody
- 分子量131 kDa
- 种属反应性Human,Mouse
- 验证应用ICC,IHC-P,FC,WB
- 抗体类型兔多抗
- 免疫原Peptide
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze/thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified
- 亚细胞定位Cytoskeleton. Nucleus. Cytosol.
- 其它名称HEP-NOS antibody
Hepatocyte NOS antibody
HEPNOS antibody
inducible antibody
Inducible nitric oxide synthase antibody
Inducible NO synthase antibody
Inducible NOS antibody
iNOS antibody
MAC NOS antibody
Macrophage NOS antibody
Nitric oxide synthase 2 inducible antibody
Nitric oxide synthase 2 inducible macrophage antibody
nitric oxide synthase 2A (inducible, hepatocytes) antibody
Nitric oxide synthase antibody
Nitric oxide synthase inducible antibody
nitric oxide synthase, macrophage antibody
NOS 2 antibody
NOS antibody
Nos II antibody
NOS type II antibody
nos2 antibody
NOS2_HUMAN antibody
NOS2A antibody
NOS2A, Inducible, Hepatocyte antibody
Peptidyl-cysteine S-nitrosylase NOS2 antibody
more
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应用
WB: 1:500
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
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Fig1: ICC staining iNOS in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig2: ICC staining iNOS in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-iNOS antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-iNOS antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-iNOS antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of LOVO cells with iNOS antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
