Anti-Smad4 antibody
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概述
- 产品描述Smad proteins, the mammalian homologs of the Drosophila Mothers against dpp (Mad) have been implicated as downstream effectors of TGFβ/BMP signaling. Smad1 (also designated Madr1 or JV4-1), Smad5 and mammalian Smad8 (also designated Smad9 or MADH6) are effectors of BMP2 and BMP4 function while Smad2 (also designated Madr2 or JV18-1) and Smad3 are involved in TGFβ and activin-mediated growth modulation. Smad4 (also designated DPC4) has been shown to mediate all of the above activities through interaction with various Smad family members. Smad6 and Smad7 regulate the response to activin/TGFβ signaling by interfering with TGFβ-mediated phosphorylation of other Smad family members.
- 产品名称Anti-Smad4 antibody
- 分子量60 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Peptide
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified
- 亚细胞定位Cytoplasm. Nucleus.
- 其它名称(Small) Mothers Against Decapentaplegic antibody
Deleted in Pancreatic Carcinoma 4 antibody
Deleted in Pancreatic Carcinoma antibody
Deleted in pancreatic carcinoma locus 4 antibody
Deletion target in pancreatic carcinoma 4 antibody
DPC 4 antibody
DPC4 antibody
hSMAD4 antibody
JIP antibody
MAD homolog 4 antibody
MAD mothers against decapentaplegic Drosophila homolog 4 antibody
MAD mothers against decapentaplegic homolog 4 antibody
MADH 4 antibody
MADH4 antibody
Med antibody
Medea antibody
Mothers against decapentaplegic homolog 4 antibody
Mothers against decapentaplegic, Drosophila, homolog of, 4 antibody
Mothers against DPP homolog 4 antibody
MYHRS antibody
OTTHUMP00000163548 antibody
SMA- and MAD-related protein 4 antibody
SMAD 4 antibody
SMAD family member 4 antibody
SMAD mothers against DPP homolog 4 antibody
SMAD4 antibody
SMAD4_HUMAN antibody
more
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应用
ICC: 1:50-1:200
IHC-P: 1:50-1:100
FC: 1:50-1:100
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Fig1: ICC staining Smad4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig2: ICC staining Smad4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Smad4 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-Smad4 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Smad4 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Smad4 antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of HUVEC cells with Smad4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary
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