Anti-ADAMTS7(NT) antibody
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概述
- 产品描述Metalloprotease that may play a role in the degradation of COMP.
- 产品名称Anti-ADAMTS7(NT) antibody
- 分子量158 KDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from rat ADAMTS7 241-340/1595
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Secreted, extracellular space, extracellular matrix. Note=Also found associated with the external cell surface.
- 其它名称A disintegrin and metalloproteinase with thrombospondin motifs 7
ADAM metallopeptidase with thrombospondin type 1 motif 7
A disintegrin and metalloprotease with thrombospondin motifs 7 preproprotein
A disintegrin and metalloproteinase with thrombospondin motifs 7
A disintegrin like and metalloprotease (reprolysin type) with thrombospondin type 1 motif 7
A disintegrin like and metalloprotease with thrombospondin type 1 motif 7
ADAM metallopeptidase with thrombospondin type 1 motif 7
ADAM metallopeptidase with thrombospondin type 1 motif 7 preproprotein
ADAM TS 7
ADAM TS7
ADAMTS 7
ADAMTS7
COMPase
DKFZp434H204
ATS7_MOUSE.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Tissue/cell: rat brain tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
Incubation: Anti-ADAMTS7 Polyclonal Antibody, Unconjugated1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining
Fig2: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (ADAMTS7(NT)) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
Fig3: Sample:
Cerebrum (Mouse) Lysate at 40 ug
Primary: Anti- ADAMTS7(NT) at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 158 kD
Observed band size: 158 kD
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
