Anti-EB2 antibody

Anti-EB2 antibody

• 概述

• 产品描述May be involved in microtubule polymerization, and spindle function by stabilizing microtubules and anchoring them at centrosomes. May play a role in cell migration.
• 产品名称Anti-EB2 antibody
• 分子量36 KDa
• 种属反应性Human,Mouse,Rat,Dog,Cow,Horse
• 验证应用WB,IHC-P
• 抗体类型兔多抗
• 免疫原KLH conjugated synthetic peptide derived from human End binding protein 2 101-200/327
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
• 亚型IgG
• 纯化方式affinity purified by Protein A
• 亚细胞定位Cytoplasm, cytoskeleton.
• 其它名称APC binding protein EB2
  APC-binding protein EB2
  EB1
  EB2
  End binding protein 2
  End-binding protein 2
  H.sapiens mRNA for novel T cell activation protein
  MAPRE2
  MARE2_HUMAN
  Microtubule associated protein RP/EB family member 2
  Microtubule-associated protein RP/EB family member 2
  T cell activation protein EB1 family.
  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

•  

  

  Fig1: Paraformaldehyde-fixed, paraffin embedded (mouse cerebellum tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (EB2) Polyclonal Antibody, Unconjugated (ER1907-89) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) instructionsand DAB staining.

   

  

  Fig2: Sample:
  U937(Human) Cell Lysate at 30 ug
  Primary: Anti- EB2 at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 36 kD
  Observed band size: 35 kD

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