Anti-EMP-1 antibody
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概述
- 产品描述EMP1 belongs to the peripheral myelin protein 22-kDa gene family that encompasses proteins with tetra-spanning membrane domains, and has a high degree of domain structure homology with EMP2 and EMP3. Although EMP1 is reportedly downregulated in the cancer region as compared with the adjacent normal epithelium at the primary tumor site, this molecule has also been proposed as a marker of resistance to cancer therapies and related to poor prognosis. With respect to clinical implications, development of an inhibitory antibody to specifically target EMP1 to infer the potential of this molecule may be challenging because of the high expression of EMP1 in pro-metastatic cancers. The intracellular loop region of EMP1 also represents a candidate target to inhibit cancer metastasis, because EMP1-induced signal transduction is triggered by the complex formation of EMP1 and copine-III. EMP-1 may act as oncogene or suppressor gene in different cancers.
- 产品名称Anti-EMP-1 antibody
- 分子量17 KDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,IHC-P,FC
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from mouse Emp1 101-160/160
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Membrane; Multi-pass membrane protein.
- 其它名称CL-20
EMP 1
EMP1
EMP1_HUMAN
Epithelial membrane protein 1
Protein B4B
TMP
Tumor-associated membrane protein.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
FC:3ug/Test
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Fig1: Sample:
Lung (Mouse) Lysate at 40 ug
Primary: Anti-EMP-1at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 17 kD
Observed band size: 19 kD
Fig2: Blank control: A431.
Primary Antibody (green line): Rabbit Anti-EMP-1 antibody
Dilution: 3μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody: Goat anti-rabbit IgG-AF647
Dilution: 3μg /test.
Protocol
The cells were incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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