Anti-DNA Primase antibody
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概述
- 产品描述DNA primase synthesizes small ribonucleotide primers for the initiation of DNA replication. The eukaryotic primase is a heterodimer of a small and a large subunit, p49 and p58 respectively. These two subunits purify as a complex tightly bound to DNA polymerase alpha. This tight association implicates polymerase alpha as the lagging-strand DNA polymerase in replication. Uniquely, primases are able to synthesize nucleotides de novo on a template by the formation of an initial dinucleotide. DNA primase initiates synthesis with a triphosphate purine moiety at the 5'-end. After synthesis of 7-10 ribonucleotides, the primer template is translocated intramolecularly to the active site of the DNA polymerase alpha subunit. The p49 subunit of DNA primase contains the catalytic active site. The human primase subunits are 90% identical in amino acid sequence to the mouse homologs.
- 产品名称Anti-DNA Primase antibody
- 分子量59 KDa
- 种属反应性Human,Mouse,Rat,Pig
- 验证应用WB
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human DNA Primase 51-150/509
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 其它名称dJ422B11.1.1
DNA primase 58kDa subunit
DNA primase large subunit
DNA primase p58
DNA primase subunit p58
p58
Polypeptide 2A p58
PRIM2
PRIM2A
PRI2_HUMAN
Primase DNA polypeptide 2 (58kDa)
Primase polpeptide 2A (58kD)
Primase polpeptide 2A 58kDa.
more
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Fig1: Protein: K562(human) lysates at 30ug;
Primary: rabbit Anti-DNA primaseat 1:300;
Secondary: HRP conjugated Goat-Anti-rabbit IgG(bs-0295G-HRP) at 1: 5000;
Predicted band size:60 kD
Observed band size:60 kD
Fig2: Sample:
bone (mouse) Lysate at 40 ug
testis (Mouse) Lysate at 40 ug
Primary: Anti-DNA primase at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 59 kD
Observed band size: 63 kD
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
