Anti-GABPA antibody
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概述
- 产品描述This gene encodes one of three GA-binding protein transcription factor subunits which functions as a DNA-binding subunit. Since this subunit shares identity with a subunit encoding the nuclear respiratory factor 2 gene, it is likely involved in activation of cytochrome oxidase expression and nuclear control of mitochondrial function. This subunit also shares identity with a subunit constituting the transcription factor E4TF1, responsible for expression of the adenovirus E4 gene. Because of its chromosomal localization and ability to form heterodimers with other polypeptides, this gene may play a role in the Down Syndrome phenotype. Two transcript variants encoding the same protein have been found for this gene.
- 产品名称Anti-GABPA antibody
- 分子量51 KDa
- 种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Rabbit,Sheep
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human GABPA 51-150/454
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Nucleus.
- 其它名称E4TF 1A
E4TF1 60
E4TF1A
GA binding protein alpha chain
GA binding protein transcription factor alpha subunit 60kDa
GA binding protein transcription factor alpha subunit
GA-binding protein alpha chain
GABP A
GABP alpha subunit
GABP alpha subunit
GABP subunit alpha
Gabpa
GABPA_HUMAN
Human nuclear respiratory factor 2 subunit alpha
NFT 2
NFT2
NRF 2
NRF 2A
NRF2
NRF2A
Nuclear respiratory factor 2 alpha subunit
Nuclear respiratory factor 2 subunit alpha
Nuclear respiratory factor 2 subunit alpha
RCH04A07
Transcription factor E4TF1 60
Transcription factor E4TF1 60
Transcription factor E4TF1-60.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
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Fig1: Sample:
293T Cell (Human) Lysate at 30 ug
Primary: Anti- GABPA at 1/300 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 51kD
Observed band size: 51kD
Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (GABPA) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.
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