Anti-CHFR antibody
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概述
- 产品描述E3 ubiquitin-protein ligase (CHFR) that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. It acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. CHFR is probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Marked reduction of CHFR expression was detected in primary tumors and decreased CHFR expression was linked to promoter hypermethylation. Restoration of CHFR expression by treatment with the microtubule stress agent nocodazole and the methyltransferase inhibitor 5-aza-2'-deoxycytidine has been reported.
- 产品名称Anti-CHFR antibody
- 分子量~73kDa
- 种属反应性Human
- 验证应用WB,ICC
- 抗体类型兔多抗
- 免疫原peptide
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified
- 亚细胞定位Nucleus
- 其它名称
- Checkpoint with forkhead and ring finger domains antibody
- Checkpoint with forkhead and RING finger domains protein antibody
- Chfr antibody
more
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应用
WB: 1:500-1:1,000
ICC: 1:200
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Fig1: Western blot analysis on Hela cell lysates using anti-CHFR rabbit polyclonal antibodies.
Fig2: ICC staining CHFR in A431 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).
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