Anti-BECN1 antibody
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概述
- 产品描述BECN1 (Beclin 1) is a coiled-coil protein that has been implicated as an inhibitor of tumorigenesis. BECN1, which associates with Bcl-2, plays a significant role in autophagy. Autophagy is the degradation of cellular proteins in the lysosomes, and when this pathway is suppressed, cell growth is deregulated. Autophagy is controlled by the same signal transduction pathway that induces the phosphorylation of the Ribosomal Protein S6, and both are mediated via amino acids. BECN1 expression in various carcinoma cell lines such as MCF7 is low, whereas it is ubiquitously expressed in normal breast tissue. In transfected MCF7 cells, BECN1 complements autophagocytosis and, subsequently, inhibits cellular proliferation. Additionally, BECN1 shares structural similarity to the yeast autophagy gene product, Apg6, and was one of the first mammalian proteins discovered to mediate autophagy.
- 产品名称Anti-BECN1 antibody
- 分子量52 kDa
- 种属反应性Human,Mouse
- 验证应用ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Synthetic peptide (KLH-coupled) within human BECN1 C terminal.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified
- 亚细胞定位Cytoplasm, Endosome, Mitochondrion membrane Mitochondrion membrane, Endoplasmic reticulum membrane
- 其它名称
- APG6 antibody
- ATG 6 antibody
- ATG6 antibody
more
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应用
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
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Fig1: Immunocytochemical staining of N2A cells using anti-BECN1 rabbit polyclonal antibody.
Fig2: Immunocytochemical staining of Hela cells using anti-BECN1 rabbit polyclonal antibody.
Fig3: Immunocytochemical staining of MCF-7 cells using anti-BECN1 rabbit polyclonal antibody.
Fig4: Immunocytochemical staining of SHG-44 cells using anti-BECN1 rabbit polyclonal antibody.
Fig5: Immunohistochemical analysis of paraffin- embedded human lung tissue using anti-BECN1 rabbit polyclonal antibody.
Fig6: Immunohistochemical analysis of paraffin- embedded human liver cancer tissue using anti-BECN1 rabbit polyclonal antibody.
Fig7: Immunohistochemical analysis of paraffin- embedded human breast tissue using anti-BECN1 rabbit polyclonal antibody.
Fig8: Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-BECN1 rabbit polyclonal antibody.
Fig9: Immunohistochemical analysis of paraffin- embedded mouse brain tissue using anti-BECN1 rabbit polyclonal antibody.
Fig10: Immunohistochemical analysis of paraffin- embedded mouse heart tissue using anti-BECN1 rabbit polyclonal antibody.
Fig11: Flow cytometric analysis of HepG2 cells with BECN1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the secondary
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