Anti-p44/42 MAPK (Erk1/2) antibody
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概述
- 产品描述Mitogen-activated protein kinase (MAPK) signaling pathways involve two closely related MAP kinases, known as extracellular-signal-related kinase 1 (ERK 1, p44) and 2 (ERK 2, p42). Growth factors, steroid hormones, G proteincoupled receptor ligands and neurotransmitters can initiate MAPK signaling pathways. Activation of ERK 1 and ERK 2 requires phosphorylation by upstream kinases such as MAP kinasekinase (MEK), MEK kinase and Raf-1. ERK 1 and ERK 2 phosphorylation can occur at specific tyrosine and threonine sites mapping within consensus motifs that include the threonine-glutamatetyrosine motif. ERK activation leads to dimerization with other ERKs and subsequent localization to the nucleus. Active ERK dimers phosphorylate serine and threonine residues on nuclear proteins and influence a host of responses that include proliferation, differentiation, transcription regulation and development. The human ERK 1 gene maps to chromosome 16p11.2 and encodes a 379 amino acid protein that shares 83% sequence identity to ERK 2.
- 产品名称Anti-p44/42 MAPK (Erk1/2) antibody
- 分子量ERK 1: 44 kDa, ERK 2: 42 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,IP,IF
- 抗体类型兔多抗
- 免疫原Amino acids 101-172 mapping near the N-terminus of ERK 2 of human origin.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃
- 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified
- 亚细胞定位Cytoplasm, Nucleus
- 其它名称
- ERK 1 antibody
- ERK 2 antibody
- ERK-2 antibody
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应用
WB: 1:100-1:1,000
IP: 1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)
IF: 1:50-1:500
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
