Anti-CD14 antibody
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概述
- 产品描述CD14 is a glycophosphatidylinositol-anchored protein expressed on the surfaces of monocytes and plymorphonuclear leukocytes. It cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). CD14 acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response and up-regulates cell surface molecules, including adhesion molecules.
- 产品名称Anti-CD14 antibody
- 分子量39kDa
- 种属反应性Human,Mouse
- 验证应用WB,ICC,FC
- 抗体类型兔多抗
- 免疫原Synthetic peptide (KLH-coupled) within human CD14 150-210 aa.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified
- 亚细胞定位Cell membrane
- 其它名称
- CD 14 antibody
- CD_antigen=CD14 antibody
- CD14 antibody
more
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应用
WB: 1:1,000
ICC: 1:50
FC: 1:50
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Fig1: Western blot analysis on K562 cell lysates using anti-CD14 rabbit polyclonal antibody.
Fig2: ICC staining CD14 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Flow cytometric analysis of Raji cells with CD14 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
