Anti-Chromogranin-A(C-term) antibody
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概述
- 产品描述Chromogranins (secretogranins) are acidic glycoproteins that localize within secretory granules of endocrine, neuroendocrine and neuronal tissue. Family members include chromogranin A (Chr-A), chromogranin B (Chr-B, also known as secretogranin I) chromogranin C (also known as secretogranin II or Sg II), secretogranin III (Sg III or SCG3). High levels of Chr-A expression is a characteristic of neuroendocrine tumors. Pancreastatin is a peptide derived from Chr-A which inhibits insulin secretion, exocrine pancreatic secretion and gastric acid secretion. Pancreastatin exists as two forms; the major form is expressed in stomach and colon extracts. In neuroendocrine cells the level Sg II has been shown to increase four-fold in response to histamine, while levels of Chr-A and Chr-B showed little or no increase. Sg III is an acidic secretory protein expressed in neuronal and endocrine cells. In the anterior lobe of the rat pituitary gland, Sg III is present in mammotropes and thyrotropes, moderately in gonadotropes and corticotropes, though not in somatotropes. Sg III and carboxypeptidase E (CPE) bind specifically to cholesterol-rich secretory granule (SG) membranes.
- 产品名称Anti-Chromogranin-A(C-term) antibody
- 分子量86 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,IHC-P,ICC
- 抗体类型小鼠单抗
- 免疫原Peptide
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度2 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG2a
- 纯化方式Protein A purified.
- 亚细胞定位Secreted. Secretory vesicle lumen.
- 其它名称
- beta Granin antibody
- betagranin (N-terminal fragment of chromogranin A) antibody
- catestatin antibody
more
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应用
WB: 1:500
ICC: 1:50-1:200
IHC-P: 1:50-1:200
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Fig1: Western blot analysis of Chromogranin A on PC-12 using anti-Chromogranin A antibody at 1/500 dilution.
Fig2: ICC staining Chromogranin A(red) in Hela cells. The nuclear counter stain is DAPI(blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Chromogranin A(red) in LOVO cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining Chromogranin A(red) in PANC-1 cells. Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded rat small intestine tissue using anti-Chromogranin A antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded rat pancreas tissue using anti-Chromogranin A antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Chromogranin A antibody. Counter stained with hematoxylin.
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