Anti-CD133 antibody [D2-B1]

Anti-CD133 antibody [D2-B1]

• 概述

• 产品描述CD133 antigen also known as prominin-1 is a glycoprotein that in humans is encoded by the PROM1 gene. It is a member of pentaspan transmembrane glycoproteins (5-transmembrane, 5-TM), which specifically localize to cellular protrusions. While the precise function of CD133 remains unknown, it has been proposed to act as an organizer of cell membrane topology.
• 产品名称Anti-CD133 antibody [D2-B1]
• 分子量Predicted band size 97 kDa
• 种属反应性Human,Mouse
• 验证应用IHC-P,ICC
• 抗体类型小鼠单抗
• 免疫原Peptide
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Peptide affinity purified
• 亚细胞定位Endoplasmic reticulum. Plasma membrane. Cell projection.
• 其它名称
  • AC133 antibody
  • Antigen AC133 antibody
  • CD133 antibody
  more

• 应用

  ICC: 1:100-1:500
  IHC-P: 1:50-1:200

•  

  

  Fig1: ICC staining CD133 (green) in LOVO cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig2: ICC staining CD133 (green) in N2A cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CD133 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CD133 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-CD133 antibody. Counter stained with hematoxylin.

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