Anti-ADAP1 antibody [3E2D9]

Anti-ADAP1 antibody [3E2D9]

• 概述

• 产品描述ADAP1 (ArfGAP With Dual PH Domains 1) is a Protein Coding gene. Among its related pathways are Class I PI3K signaling events and B Cell Receptor Signaling Pathway (sino). GO annotations related to this gene include GTPase activator activity and inositol 1,3,4,5 tetrakisphosphate binding. An important paralog of this gene is ADAP2.
• 产品名称Anti-ADAP1 antibody [3E2D9]
• 分子量43.4kDa
• 种属反应性Human,Rat
• 验证应用WB,FC
• 抗体类型小鼠单抗
• 免疫原Purified recombinant fragment of human ADAP1 (AA: 240-370) expressed in E. Coli.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS with 0.05% sodium azide.
• 亚型IgG1
• 纯化方式Protein G purified.
• 亚细胞定位Cytoplasmic, Nuclear and Plasma membrane
• 其它名称
  • ArfGAP with dual PH domains 1 antibody
  • CENTA 1 antibody
  • CENTA1 antibody
  more

• 应用

  WB: 1:500-1:2,000
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of ADAP1 against human ADAP1 (AA: 240-370) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody  was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

   

  

  Fig2: Western blot analysis of ADAP1 against HEK293 (1) and ADAP1 (AA: 240-370)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody  was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

   

  

  Fig3: Western blot analysis of ADAP1 against C6 (1), A549 (2), and HepG2 (3) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

   

  

  Fig4: Flow cytometric analysis of ADAP1 was done on K562 cells. The cells were fixed, permeabilized and stained with the primary antibody (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

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