Anti-Ubiquitin antibody [6G6]

Anti-Ubiquitin antibody [6G6]

• 概述

• 产品描述Exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling.
• 产品名称Anti-Ubiquitin antibody [6G6]
• 种属反应性Human, Rat, Mouse
• 验证应用WB, IHC-P
• 抗体类型小鼠单抗
• 免疫原Synthetic Peptide of Ubiquitin
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
• 纯化方式Immunogen affinity purified.
• 亚细胞定位Nucleus

• 应用

  WB: 1:1000-1:2000
  IHC-P: 1:100-200

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  Fig1: Western blot analysis of 1) Hela Cell Lysate, 2) 3T3 Cell Lysate, 3) Rat Brain Tissue Lysate using Ubiquitin Mouse mAb diluted at 1:1000.

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded Human Breast CarcinomaTissue using Ubiquitin Mouse mAb diluted at 1:200.

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