Anti-RALA antibody
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概述
- 产品描述Ral A and Ral B constitute a distinct subfamily of Ras-related GTPases (i.e., GDP/GTP binding proteins). Ral proteins are activated by a unique nucleotide exchange factor, Ral GDS, and deactivated by a distinct GTPase-activating protein. Unlike Ras proteins, Ral A and Ral B fail to induce transformed foci when activated variants are expressed in various recipient cells. A potential downstream target of Ral, designated Ral BP-1, has been shown to contain a Rho-GTPase-activating domain. This Rho-GTPase-activating domain interacts preferentially with the Rho family member Cdc42. A Ras/Ral signaling pathway has been reported to mediate phospholipase D (PLD) activation by v-Src, thus indicating PLD as another downstream target of Ral A.
- 产品名称Anti-RALA antibody
- 分子量24 kDa
- 种属反应性Human,Mouse,Monkey
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型小鼠单抗
- 免疫原Recombinant protein
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度2 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG1
- 纯化方式Protein A purified.
- 亚细胞定位Cell surface. Cell membrane. Cleavage furrow.
- 其它名称
- MGC48949 antibody
- Ral a antibody
- Ral A protein antibody
more
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应用
WB: 1:500-1:2,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:100-1:200
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Fig1: Western blot analysis of RALA on human RALA recombinant protein using anti-RALA antibody at 1/1,000 dilution.
Fig2: Western blot analysis of RALA on HEK293 (1) and RALA-hIgGFc transfected HEK293 (2) cell lysate using anti-RALA antibody at 1/1,000 dilution.
Fig3: Western blot analysis of RALA on different cell lysate using anti-RALA antibody at 1/1,000 dilution.
Positive control:
Lane 1: HepG2
Lane 2: MCF-7
Lane 3: A549
Lane 4: K562
Lane 5: Raji
Lane 6: MOLT4
Lane 7: Hela
Lane 8: COS7
Lane 9: NIH/3T3
Fig4: ICC staining RALA (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-RALA antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-RALA antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of MCF-7 cells with RALA antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
