Anti-Synaptophysin antibody [A1-7]

Anti-Synaptophysin antibody [A1-7]

• 概述

• 产品描述Synaptophysin is a 38 kDa glycoprotein present in the membrane of neuronal presynaptic vesicles in brain, spinal cord, retina, vesicles of adrenal medulla, neuromuscular junctions, and endocrine cells. It is also expressed by neuroendocrine cells throughout the body, both normal and neoplastic. Synaptophysin is a useful marker for the identification of normal neuroendocrine cells and neuroendocrine neoplasms.
• 产品名称Anti-Synaptophysin antibody [A1-7]
• 分子量34kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Synthetic peptide (KLH-coupled) within human Synaptophysin 220-280 aa.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Peptide affinity purified
• 亚细胞定位Cytoplasmic vesicle. Membrane. Synapse. Synaptosome.
• 其它名称
  • Major synaptic vesicle protein p38 antibody
  • MRX96 antibody
  • MRXSYP antibody
  more

• 应用

  WB: 1:200-1:1,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of Synaptophysin on mouse brain tissue lysate using anti-Synaptophysin antibody at 1/2,000 dilution.

   

  

  Fig2: ICC staining Synaptophysin (red) in PANC-1 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded rat pancreas cancer tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.

   

  

  Fig5: Flow cytometric analysis of SHG-44 cells with Synaptophysin antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.

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