Anti-CRISPR-Cas9 antibody [8-A6-6]

Anti-CRISPR-Cas9 antibody [8-A6-6]

• 概述

• 产品描述The Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Type II system is currently the most commonly used RNA-Guided Endonuclease technology for genome engineering. There are two distinct components to this system: (1) a guide RNA and (2) an endonuclease, in this case the CRISPR associated (Cas) nuclease, Cas9. The gRNA/Cas9 complex is recruited to the target sequence by the base-pairing between the gRNA sequence and the complement to the target sequence in the genomic DNA. For successful binding of Cas9, the genomic target sequence must also contain the correct Protospacer Adjacent Motiff (PAM) sequence immediately following the target sequence.
• 产品名称Anti-CRISPR-Cas9 antibody [8-A6-6]
• 种属反应性Human
• 验证应用WB, ICC
• 抗体类型小鼠单抗
• 免疫原recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgM
• 纯化方式Protein A purified.
• 其它名称
  • Cas9 antibody
  • CRISPR-associated endonuclease Cas9/Csn1 antibody
  • CRISPR-Cas9/Csn1 antibody
  more

• 应用

  ELISA:
  WB: 1:5,000
  IP:
  ICC: 1:200
  IF:
  IHC-P:
  FC:

•  

  

  Fig1: Western blot analysis of CRISPR-Cas9 on 293T cell lysates using anti-CRISPR-Cas9 antibody at 1/5000 dilution. Lane 1: CRISPR-Cas9 transfected 293 cells lysate Lane 2: Non-transfected 293 cells lysate

   

  

  Fig2: Immunofluorescence staining CRISPR-Cas9 in CRISPR-Cas9 transfected 293T cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

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