Anti-DNMT3L antibody [B0-5]

Anti-DNMT3L antibody [B0-5] 

• 概述

• 产品描述DNA (cytosine-5)-methyltransferase 3-like is an enzyme that in humans is encoded by the DNMT3L gene. CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a nuclear protein with similarity to DNA methyltransferases. This protein is not thought to function as a DNA methyltransferase as it does not contain the amino acid residues necessary for methyltransferase activity. However, this protein does stimulate de novo methylation by DNA cytosine methyltransferase 3 alpha and it is thought to be required for the establishment of maternal genomic imprints. This protein also mediates transcriptional repression through interaction with histone deacetylase 1.
• 产品名称Anti-DNMT3L antibody [B0-5]
• 分子量44kDa
• 验证应用WB,ICC,IHC-P
• 抗体类型小鼠单抗
• 免疫原recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein G purified.
• 亚细胞定位Nucleus
• 其它名称
  • Cytosine 5 methyltransferase 3 like protein antibody
  • DNA (cytosine 5 ) methyltransferase 3 like antibody
  • DNA (cytosine-5)-methyltransferase 3-like antibody
  more

• 应用

  ICC: 1:200-1:500
  IHC-P: 1:200

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  Fig1: ICC staining DNMT3L in HepG2 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).

   

  

  Fig2: ICC staining DNMT3L in NCCIT cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded mouse colonic tissue using anti- DNMT3L mouse monoclonal antibody.

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