Anti-ALDH6A1 antibody [B8-H9]

Anti-ALDH6A1 antibody [B8-H9]

• 概述

• 产品描述Aldehyde dehydrogenases (ALDHs) mediate the NADP+-dependent oxidation of aldehydes into acids and play an important role in the detoxification of alcohol-derived acetaldehyde, as well as in lipid peroxidation and in the metabolism of corticosteroids, biogenic amines and neurotransmitters. ALDH6A1 (aldehyde dehydrogenase family 6 member A1), also known as MMSDH or MMSADHA, is a 535 amino acid mitochondrial protein that belongs to the aldehyde dehydrogenase family. Considered a mitochondrial methylmalonate semialdehyde dehydrogenase, ALDH6A1 catalyzes the irreversible oxidative decarboxylation of malonate and methylmalonate semialdehydes to acetyl- and propionyl-CoA. It is suggested that ALDH6A1 plays a role in the valine and pyrimidine catabolic pathways.
• 产品名称Anti-ALDH6A1 antibody [B8-H9]
• 分子量58 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Mitochondrion. Nucleoplasm.
• 其它名称
  • 1110038I05Rik antibody
  • AI314632 antibody
  • Aldehyde dehydrogenase 6 family member A1 antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of ALDH6A1 on human ALDH6A1 recombinant protein using anti-ALDH6A1 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of ALDH6A1 on HEK293 (1) and ALDH6A1-hIgGFc transfected HEK293 (2) cell lysate using anti-ALDH6A1 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of ALDH6A1 on different cell lysate using anti-ALDH6A1 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: Jurkat
  Lane 2: HEK293
  Lane 3: Hela
  Lane 4: MCF-7
  Lane 5: LNcap

   

  

  Fig4: ICC staining ALDH6A1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-ALDH6A1 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti-ALDH6A1 antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of Hela cells with ALDH6A1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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