Anti-IL3RA antibody [C5-E11]

Anti-IL3RA antibody [C5-E11]

• 概述

• 产品描述Interleukin-3, or IL-3, is a pleiotropic cytokine that is primarily secreted by activated T lymphocytes and stimulates the proliferation and differentiation of hematopoietic cells. IL-3 exerts its biological effects through a receptor which consists of a ligand-specific α subunit (IL-3Rα) and a signal transducing β subunit (IL-3Rβ) common to the IL-3/IL-5/GM-CSF receptors. The α subunits are low-affinity ligand-binding proteins while the β subunits do not themselves bind ligand, but are required for high affinity binding by the α subunits. The mouse IL-3 receptor has two distinct β subunits, one that functions only in IL-3-mediated cell signaling and a second that is shared with IL-5 and GM-CSF. The murine β subunits are 91% homologous at the amino acid level but only 56% homologous to the human β subunit. The carboxy-terminus of the β subunit has been shown to be necessary for activation of the MAP kinase signaling pathway. Although the IL-3 receptor has no intrinsic kinase activity, stimulation with IL-3 leads to tyrosine phosphorylation of the JAK/Tyk 2 family member, JAK2, which in turn activates and causes nuclear translocation of Stat5a and Stat5b.
• 产品名称Anti-IL3RA antibody [C5-E11]
• 分子量43 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Membrane.
• 其它名称
  • CD123 antibody
  • CD123 antigen antibody
  • hIL 3Ra antibody
  more

• 应用

  WB: 1:500-1:1,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of IL3RA on human IL3RA recombinant protein using anti-IL3RA antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of IL3RA on HEK293 (1) and IL3RA-hIgGFc transfected HEK293 (2) cell lysate using anti-IL3RA antibody at 1/1,000 dilution.

   

  

  Fig3: ICC staining IL3RA (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-IL3RA antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-IL3RA antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of Hela cells with IL3RA antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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