Anti-CD22 antibody [C4-H1]

Anti-CD22 antibody [C4-H1]

• 概述

• 产品描述The B lymphocyte specific CD22 antigen, also designated B-lymphocyte cell adhesion molecule (BLCAM), sialic acid-binding Ig-like lectin 2 (Siglec-2) and Leu-14, is a type I integral membrane glycoprotein, structurally similar to other cell adhesion molecules (CAMs), which acts as a regulator of B cell signaling. CD22 is expressed as both a cytoplasmic and membrane protein during discrete stages of B cell lymphocyte differentiation. The cytoplasmic form of CD22, expressed early in B cell development, is a useful marker for acute lymphocytic leukemia. The membrane form of CD22 is expressed in mature B cells prior to their differentiation into plasma cells. Alternative splicing results in two different isoforms, CD22α and CD22β. The CD22β monomer is the principally occurring isoform but CD22 also appears as a heterodimer of CD22β and the shorter isoform, CD22α.

• 产品名称Anti-CD22 antibody [C4-H1]

• 分子量95 kDa

• 种属反应性Human

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型小鼠单抗

• 免疫原Recombinant protein

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度2 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG1

• 纯化方式Protein A purified.

• 亚细胞定位Cell membrane.

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• ​其它名称

  more

  • B cell receptor CD22 precursor antibody

  • B lymphocyte cell adhesion molecule antibody

  • B-cell receptor CD22 antibody

• 应用

  WB: 1:500-1:1,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:100-1:200

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  Fig1: Western blot analysis of CD22 on human CD22 recombinant protein using anti-CD22 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of CD22 on HEK293 (1) and CD22-hIgGFc transfected HEK293 (2) cell lysate using anti-CD22 antibody at 1/1,000 dilution.

   

  

  Fig3: ysis of CD22 on different cell lysate using anti-CD22 antibody at 1/1,000 dilution.
  Positive control: Line1: L1210 Line2: Hela Line3: HEK293 Line4: Jurkat Line5: OCM-1 Line6: A431 Line7: NIH/3T3

   

  

  Fig4: ICC staining CD22 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-CD22 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using anti-CD22 antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of Hela cells with CD22 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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