Anti-GRP78 BiP antibody [2-7]
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概述
- 产品描述Binding immunoglobulin protein (BiP) also known as 78 kDa glucose-regulated protein (GRP-78) or heat shock 70 kDa protein 5 (HSPA5) is a protein that in humans is encoded by the HSPA5 gene. BiP is a HSP70 molecular chaperone located in the lumen of the endoplasmic reticulum (ER) that binds newly synthesized proteins as they are translocated into the ER, and maintains them in a state competent for subsequent folding and oligomerization. BiP is also an essential component of the translocation machinery, as well as playing a role in retrograde transport across the ER membrane of aberrant proteins destined for degradation by the proteasome. Like many stress and heat shock proteins, BiP/GRP78 has potent immunological activity when released from the internal environment of the cell into the extracelluar space.specifically, it feeds anti-inflammatory and pro-resolutory signals into immune networks, thus helping to resolve inflammation.
- 产品名称Anti-GRP78 BiP antibody [2-7]
- 分子量78kDa
- 种属反应性Human
- 验证应用WB,ICC,IHC-P
- 抗体类型小鼠单抗
- 免疫原peptide
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度2 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG1
- 纯化方式Protein A purified.
- 亚细胞定位Cytoplasm, endoplasmic reticulum lumen
- 其它名称
- 78 kDa glucose regulated protein antibody
- 78 kDa glucose-regulated protein antibody
- AL022860 antibody
more
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应用
WB: 1:5,000-1:10,000
ICC: 1:200
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Fig1: Western blot analysis on human spermatozoa lysates using anti- GRP78 mouse mAb.
Fig2: ICC staining GRP78 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Western blot analysis of GRP78 on hybrid fish (crucian-carp) heart tissue lysate using anti-GRP78 antibody at 1/500 dilution.
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