Anti-Human IgG kapa light chain antibody [A11-G11]
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概述
- 产品描述IgG is the most abundant immunoglobulin and is approximately equally distributed in blood and in tissue liquids, constituting 75% of serum immunoglobulins in humans. There are 4 subclasses: IgG1 (66%), IgG2 (23%), IgG3 (7%) and IgG4 (4%). This is the only isotype that can pass through the human placenta, thereby providing protection to the fetus in its first weeks of life before its own immune system has developed. This antibody reacts with kappa-light-chain of human IgG.
- 产品名称Anti-Human IgG kapa light chain antibody [A11-G11]
- 分子量25 kDa
- 种属反应性Human
- 验证应用WB, ELISA
- 抗体类型小鼠单抗
- 免疫原Native protein.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度2 mg/ml.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG2a
- 纯化方式Protein A purified.
- 亚细胞定位Cell membrane, Immunoglobulin, Membrane, Secreted
- 其它名称
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- Human IgG kapa light chain antibody
- IGK@ protein antibody
- IGK@ antibody
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应用
WB: 1:2,000-1:5,000
ELISA: 1:5,000
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Fig1: Western blot analysis of Human IgG kapa light chain on human IgG protein lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody ) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody (HA1006) at 1:5,000 dilution was used for 1 hour at room temperature.
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