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| 别名: | FGF2,FGF-2,BFGF,FGFB,FGFβ,FGF-β,FGF basic,HBGF-2,HBGF2 | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Human FGF basic Protein, Tag Free
FGF2,FGF-2,BFGF,FGFB,FGFβ,FGF-β,FGF basic,HBGF-2,HBGF2
Human FGF basic, Tag Free is expressed from E.coli cells. It contains AA Pro 143 - Ser 288
Predicted N-terminus: Met
Request for sequence
This protein carries no "tag".
The protein has a calculated MW of 16.5 kDa. The protein migrates as 17 kDa under reducing (R) condition (SDS-PAGE).
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human FGF basic, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human FGF basic, Tag Free at 2 μg/mL (100 μL/well) can bind Human Glypican 3, Fc Tag, low endotoxin
Protocol
Immobilized Human FGF basic, Tag Freeat 1 μg/mL (100 μL/well) can bind Human Glypican 1, Fc Tag ) with a linear range of 1-39 ng/mL (Routinely tested).
Protocol
Immobilized Human FGF basic, Tag Freen CM5 Chip can bind Heparin with an affinity constant of 8.37 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Loaded Biotinylated Heparin on SA Biosensor, can bind Human FGF basic, Tag Free with an affinity constant of 6.27 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Human FGF basic, Tag Free stimulates proliferation of HUVEC in the range of 0-20 ng/mL. The EC50 for this effect is 0.416-0.630 ng/mL (Routinely tested).
