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Human VEGF-C / Flt4-L Protein, His Tag (MALS verified)

Human VEGF-C / Flt4-L Protein, His Tag (MALS verified)
别名: VEGFC,Flt4-L,VRP
储存条件: -20℃ 
Human VEGF-C / Flt4-L Protein, His Tag (MALS verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-VEC-H4225-50ug 50g 现货 2500.00 2500
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熔点:
密度:
储存条件: -20℃

 

Human VEGF-C / Flt4-L Protein, His Tag (MALS verified)

分子别名(Synonym)

VEGFC,Flt4-L,VRP

表达区间及表达系统(Source)

Human VEGF-C, His Tag is expressed from human 293 cells. It contains AA Thr 103 - Arg 227 .

Predicted N-terminus: Thr 103

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 14.9 kDa. The protein migrates as 19-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human VEGF-C, His Tag (Cat. No. VEC-H4225) SDS-PAGE gel

Human VEGF-C, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Human VEGF-C, His Tag is more than 95% and the molecular weight of this protein is around 32-42 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human VEGF R3, Fc Tag  at 5 μg/mL (100 μL/well) can bind Human VEGF-C, His Tagwith a linear range of 2-20 ng/mL (QC tested).

Protocol

活性(Bioactivity)-SPR

Biotinylated Human SPR

Human VEGF-C, His Tag captured on CM5 Chip via anti-His antibody can bind Human VEGF R3, Fc Tag  with an affinity constant of 41.1 pM as determined in SPR assay (Biacore T200) (Routinely tested).

 

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