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| 别名: | IL-23 alpha & IL-12 beta | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Biotinylated Human IL-23 alpha&IL-12 beta Heterodimer Protein, His,Avitag™&Tag Free (MALS verified)
IL-23 alpha & IL-12 beta
Biotinylated Human IL-23A&IL-12B Heterodimer Protein, His,Avitag&Tag Free is expressed from human 293 cells . It contains AA Arg 20 - Pro 189 (IL23A) & Ile 23 - Ser 328 (IL12B).
Predicted N-terminus: His (IL23A) & Ile 23 (IL12B)
Request for sequence
Biotinylated Human IL-23A&IL-12B Heterodimer Protein, His,Avitag&Tag Free, produced by co-expression of IL23A and IL12B, has a calculated MW of 21.9 kDa (IL23A) & 34.7 kDa (IL12B). Subunit IL23A is fused with an Avi tag (Avitag™), followed by a polyhistidine tag at the N-terminus and subunit IL12B contains no tag. The predicted N-terminus is His (IL23A) & Ile 23 (IL12B). The reducing (R) protein migrates as 26 kDa (IL23A) & 40-45 kDa (IL12B) respectively due to glycosylation.
Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Passed as determined by the HABA assay / binding ELISA.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Biotinylated Human IL-23A&IL-12B Heterodimer Protein, His,Avitag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Biotinylated Human IL-23A&IL-12B Heterodimer Protein, His,Avitag&Tag Free is more than 85% and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.
Report
Immobilized Anti-p19 subunit of IL23 (Anti-IL23A) MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-23A&IL-12B Heterodimer Protein, His,Avitag&Tag Free with a linear range of 1.2-19.5 ng/mL (QC tested).
Protocol
Immobilized Anti-p40 subunit of IL23 (Anti-IL23B) MAb, Human IgG1 at 2 μg/mL (100 μL/well) can bind Biotinylated Human IL-23A&IL-12B Heterodimer Protein, His,Avitag&Tag Free with a linear range of 4.9-39 ng/mL (Routinely tested).
Protocol
Immobilized Biotinylated Human IL-23A&IL-12B Heterodimer Protein, His,Avitag&Tag Free at 2 μg/mL (100 μL/well) on streptavidin precoated (0.2 μg/well) plate, can bind Human IL-23 R, Fc Tagwith a linear range of 0.4-6 ng/mL (Routinely tested).
Protocol
Captured Biotinylated Human IL-23A&IL-12B Heterodimer Protein, His,Avitag&Tag Free on Biotin CAP - Series S sensor Chip can bind Human IL-23 R, His Tag affinity constant of 4.77 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Loaded Biotinylated Human IL-23A&IL-12B Heterodimer Protein, His,Avitag&Tag Free on SA Biosensor, can bind Human IL-23 R, His Tag (SPR verified) with an affinity constant of 33.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Biotinylated Human IL-23A&IL-12B Heterodimer Protein, His,Avitag&Tag Free stimulates secretion of IL-17 by mousespleen cells. The ED50 for this effect is 0.191-0.2173 ng/mL (Routinely tested).
Protocol
Cell based assay shows that the secretion of IL-17 induced by Biotinylated Human IL-23A&IL-12B Heterodimer Protein, His,Avitag&Tag Free is inhibited by increasing concentration of the anti-human IL-23 neutralizing antibody. The IC50 is between 0.25-1.40 ng/mL (Routinely tested).
