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Human IL-1RL1 / ST2 Protein, Fc Tag

Mouse IL-Human IL-1RL1 / ST2 Protein, Fc Tag1RL1 / ST2 Protein, Fc Tag
别名: Mouse IL-Human IL-1RL1 / ST2 Protein, Fc Tag1RL1 / ST2 Protein, Fc Tag
储存条件: -20℃ 
Human IL-1RL1 / ST2 Protein, Fc Tag
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-IL1-H5250-200ug 200ug 现货 2800.00 2800
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熔点:
密度:
储存条件: -20℃

 

Mouse IL-Human IL-1RL1 / ST2 Protein, Fc Tag1RL1 / ST2 Protein, Fc Tag

分子别名(Synonym)

IL1RL1,DER4,FIT-1,IL33R,ST2,ST2L,ST2V,T1

表达区间及表达系统(Source)

Human IL-1RL1, Fc Tag is expressed from human 293 cells. It contains AA Lys 19 - Pro 323 .

Predicted N-terminus: Lys 19

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 61.0 kDa. The protein migrates as 80-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human IL-1RL1, Fc Tag (Cat. No. IL1-H5250) SDS-PAGE gel

Human IL-1RL1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human IL-1RL1, Fc Tagat 5 μg/mL (100 μL/well) can bind Human IL-33, His Tag with a linear range of 5-40 ng/mL (QC tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Human IL-1RL1, Fc Tag on Protein A Biosensor, can bind with Biotinylated Human IL-33, His,Avitag  an affinity constant of 1.81 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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