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Mouse IL-10 Protein, His Tag

Mouse IL-10 Protein, His Tag
别名: CSIF,IL-10,IL10A,TGIF,B-TCGF,GVHDS,MGC126450,MGC126451,RP11-262N9.1,Interleukin-10
储存条件: -20℃ 
Mouse IL-10 Protein, His Tag
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-IL0-M4248-20ug 20ug 现货 1600.00 1600
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熔点:
密度:
储存条件: -20℃

 

Mouse IL-10 Protein, His Tag

分子别名(Synonym)

CSIF,IL-10,IL10A,TGIF,B-TCGF,GVHDS,MGC126450,MGC126451,RP11-262N9.1,Interleukin-10

表达区间及表达系统(Source)

Mouse IL-10, His Tag  is expressed from human 293 cells . It contains AA Ser 19 - Ser 178.

Predicted N-terminus: His

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 19.6 kDa. The protein migrates as 22-25 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>92% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Mouse IL-10, His Tag (Cat. No. IL0-M4248) SDS-PAGE gel

Mouse IL-10, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 92%.

活性(Bioactivity)-Cell based assay

Biotinylated Human CELL_BASE

Mouse IL-10, His Tag  inhibits LPS-induced secretion of IL-6 by RAW264.7 cells. The EC50 for this effect is 0.27-0.39 ng/mL (Routinely tested).

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