Biotinylated Human IL-23 R Protein, Fc,Avitag™

Biotinylated Human IL-23 R Protein, Fc,Avitag™

分子别名（Synonym）

IL-23 R,IL-23 Receptor

表达区间及表达系统（Source）

Biotinylated Human IL-23 R, Fc,Avitag  is expressed from human 293 cells. It contains AA Gly 24 - Gly 355.

Predicted N-terminus: Gly 24

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 66.3 kDa. The protein migrates as 95-105 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记（Biotinylation）

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度（Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳（SDS-PAGE）

Biotinylated Human IL-23 R, Fc,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性（Bioactivity）-ELISA

Immobilized Biotinylated Human IL-23 R, Fc,Avitag  at 5 μg/mL (100 μL/well) on Streptavidin precoated (0.5 μg/well) plate can bind Human IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free  with a linear range of 39-156 ng/mL (QC tested).

Protocol

Immobilized Biotinylated Human IL-23 R, Fc,Avitag  at 5 μg/mL (100 μL/well), can bind pre-mixed increasing concentrations of Human IL23A/p19 Neutralizing Antibody and 0.25 μg/mL (100 μL/well) Human IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free with a half maximal inhibitory concentration (IC50) of 0.3889 μg/mL (Routinely tested).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！