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Human VEGF121 Protein, His Tag (MALS verified)

Human VEGF121 Protein, His Tag (MALS verified)
别名: RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF
储存条件: -20℃ 
Human VEGF121 Protein, His Tag (MALS verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-VE1-H5246-50ug 50ug 现货 3400.00 3400
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熔点:
密度:
储存条件: -20℃

 

Human VEGF121 Protein, His Tag (MALS verified)

分子别名(Synonym)

RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF

表达区间及表达系统(Source)

Human VEGF121, His Tag  is expressed from human 293 cells . It contains AA Ala 27 - Arg 147 

Predicted N-terminus: His

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 14.9 kDa. As a result of glycosylation, the protein migrates as 16 kDa and 18-20 kDa under reducing (R) condition, and 30 kDa and 32-40 kDa under non-reducing (NR) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by reduced SDS-PAGE.

>90% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human VEGF121, His Tag (Cat. No. VE1-H5246) SDS-PAGE gel

Human VEGF121, His Tag on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Human VEGF121, His Tag is more than 90% and the molecular weight of this protein is around 36-46 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human VEGF121, His Tag at 1 μg/mL (100 μL/well) can bind Human VEGFR1/R2, Fc Tag with a linear range of 0.4-6 ng/mL (QC tested).

 

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