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| 别名: | EGFR,ERBB,ERBB1,HER1,PIG61,mENA | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Human EGF R Protein, His Tag, low endotoxin (MALS verified)
EGFR,ERBB,ERBB1,HER1,PIG61,mENA
Human EGF R, His Tag, low endotoxin is expressed from human 293 cells. It contains AA Leu 25 - Ser 645
Predicted N-terminus: Leu 25
Request for sequence
This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 70.5kDa. The protein migrates as 77-104 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 0.01 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human EGF R, His Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human EGF R, His Tag, low endotoxin is more than 90% and the molecular weight of this protein is around 77-104 kDa verified by SEC-MALS.
Report
Immobilized Cetuximab at 2 μg/mL (100 μL/well) can bind Human EGF R, His Tag, low endotoxin with a linear range of 0.8-6 ng/mL (QC tested).
Protocol
Immobilized Erbitux on CM5 Chip via anti-human Fc IgG, can bind Human EGF R, His Tag, low endotoxin with an affinity constant of 0.492 nM as determined in SPR assay (Biacore T200) ( Routinely tested).
