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Human TNFSF11 / RANKL / CD254 Protein, Mouse IgG2a Fc Tag

Human TNFSF11 / RANKL / CD254 Protein, Mouse IgG2a Fc Tag
别名: RANKL,CD254,TRANCE,OPGL,ODF
储存条件: -20℃ 
Human TNFSF11 / RANKL / CD254 Protein, Mouse IgG2a Fc Tag
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-RAL-H52A6-50ug 50ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

Human TNFSF11 / RANKL / CD254 Protein, Mouse IgG2a Fc Tag

分子别名(Synonym)

RANKL,CD254,TRANCE,OPGL,ODF

表达区间及表达系统(Source)

Human TNFSF11, Mouse IgG2a Fc Tag is expressed from human 293 cells. It contains AA Gly 64 - Asp 245

Predicted N-terminus: Glu

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a mouse IgG2a Fc tag at the N-terminus.

The protein has a calculated MW of 47.4 kDa. The protein migrates as 55-60 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human TNFSF11, Mouse IgG2a Fc Tag (Cat. No. RAL-H52A6) SDS-PAGE gel

Human TNFSF11, Mouse IgG2a Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

获取HPLC数据

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human Osteoprotegerin, His Tagat 2 μg/mL (100 μL/well) can bind Human TNFSF11, Mouse IgG2a Fc Tag with a linear range of 0.2-2 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Human TNFSF11, Mouse IgG2a Fc Tagat 2 μg/mL (100 μL/well) can bind Anti-Human TNFSF11 Antibody, Human IgG1 with a linear range of 0.2-2 ng/mL (Routinely tested).

 

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