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Biotinylated Human GITR / TNFRSF18 Protein, His,Avitag™ (MALS verified)

Biotinylated Human GITR / TNFRSF18 Protein, His,Avitag™ (MALS verified)
别名: AITR,GITR,TNFRSF18,CD357
储存条件: -20℃ 
Biotinylated Human GITR / TNFRSF18 Protein, His,Avitag™ (MALS verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-GIR-H82E1-25ug 25ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

Biotinylated Human GITR / TNFRSF18 Protein, His,Avitag™ (MALS verified)

分子别名(Synonym)

AITR,GITR,TNFRSF18,CD357

表达区间及表达系统(Source)

Biotinylated Human GITR, His,Avitag™  is expressed from human 293 cells . It contains AA Gln 26 - Glu 161 

Predicted N-terminus: Gln 26

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

The protein has a calculated MW of 18.1 kDa. The protein migrates as 22-26 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

>95% as determined by SEC-MALS.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Biotinylated Human GITR, His,Avitag™ (Cat. No. GIR-H82E1) SDS-PAGE gel

Biotinylated Human GITR, His,Avitag™ on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

The purity of Biotinylated Human GITR, His,Avitag™ is more than 95% and the molecular weight of this protein is around 20-30 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human GITR Ligand, Fc Tag  at 10 μg/mL (100 μL/well) can bind Biotinylated Human GITR, His,Avitag with a linear range of 0.2-8 ng/mL (QC tested).

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