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Biotinylated Human G-CSF R / CD114 Protein, Avitag™,His Tag (MALS verified)

Biotinylated Human G-CSF R / CD114 Protein, Avitag™,His Tag (MALS verified)
别名: CSF3R,CD114,GCSFR
储存条件: -20℃ 
Biotinylated Human G-CSF R / CD114 Protein, Avitag™,His Tag (MALS verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-GCR-H82E4-25ug 25ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

Biotinylated Human G-CSF R / CD114 Protein, Avitag™,His Tag (MALS verified)

分子别名(Synonym)

CSF3R,CD114,GCSFR

表达区间及表达系统(Source)

Biotinylated Human G-CSF R, Avitag,His Tag is expressed from human 293 cells . It contains AA Glu 25 - Pro 621 .

Predicted N-terminus: Glu 25

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries an Avi tag (Avitag™) at the C-terminus, followed by a polyhistidine tag.

The protein has a calculated MW of 69.2 kDa. The protein migrates as 85-100 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

标记(Biotinylation)

Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.

蛋白标记度(Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Biotinylated Human G-CSF R, Avitag,His Tag (Cat. No. GCR-H82E4) SDS-PAGE gel

Biotinylated Human G-CSF R, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Biotinylated Human G-CSF R, Avitag,His Tag  is more than 85% and the molecular weight of this protein is around 148-184 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized ActiveMax® Human G-CSF, Tag Free at 2 μg/mL (100 μL/well) can bind Biotinylated Human G-CSF R, Avitag,His Tagwith a linear range of 0.01-0.6 μg/mL (QC tested).

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