Anti-Jagged 1 antibody

Anti-Jagged 1 antibody

• 概述

• 产品描述The LIN-12/Notch family of transmembrane receptors is believed to play a central role in development by regulating cell fate decisions. Ligands for Notch include Jagged1, Jagged2 and Delta. Jagged is a membrane protein and can activate Notch and prevent myoblast differentiation by inhibiting the expression of muscle regulatory and structural genes. It is involved in mammalian cardiovascular development and in cell-fate decisions during hematopoiesis. Jagged is expressed in adult and fetal tissues, and expression is upregulated in cervical squamous cell carcinoma. Familial Tetralogy of Fallot, the most common form of complex congenital heart disease, is caused by a mutation in the Jagged1 gene.
• 产品名称Anti-Jagged 1 antibody
• 分子量133 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用ICC,IHC-P,FC
• 抗体类型兔多抗
• 免疫原Peptide
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG
• 纯化方式Peptide affinity purified
• 亚细胞定位Membrane.
• 其它名称
  • AGS antibody
  • AHD antibody
  • AWS antibody
  more

• 应用

  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:200

•  

  

  Fig1: ICC staining Jagged 1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig2: ICC staining Jagged 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining Jagged 1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Jagged 1 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Jagged 1 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of HepG2 cells with Jagged 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

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