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Human Latent TGF-beta 1 / TGFB1 Protein, His Tag (MALS verified)

Human Latent TGF-beta 1 / TGFB1 Protein, His Tag (MALS verified)
别名: Latent TGF-beta 1,Latent TGFB1,TGFB1,CED,DPD1,LAP,TGF-beta-1,TGFB
储存条件: -20℃ 
Human Latent TGF-beta 1 / TGFB1 Protein, His Tag (MALS verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-TG1-H524x-50ug 50ug 现货 2680.00 2680
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熔点:
密度:
储存条件: -20℃

 

Human Latent TGF-beta 1 / TGFB1 Protein, His Tag (MALS verified)

 

分子别名(Synonym)

Latent TGF-beta 1,Latent TGFB1,TGFB1,CED,DPD1,LAP,TGF-beta-1,TGFB

表达区间及表达系统(Source)

Human Latent TGFB1, His Tag is expressed from human 293 cells . It contains AA Leu 30 - Ser 390.

Predicted N-terminus: His

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 42.6 kDa (monomer). The protein migrates as 37-45 kDa (LAP) and 13 kDa (TGFB1) under reducing (R) condition (SDS-PAGE) due to glycosylation and Interchain disulfide bond.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human Latent TGFB1, His Tag (Cat. No. TG1-H524x) SDS-PAGE gel

Human Latent TGFB1, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Human Latent TGFB1, His Tag is more than 85% and the molecular weight of this protein is around 100-110 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human Latent TGFB1, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV&ITGB6 Heterodimer Protein, His,Avitag&Tag Free  with a linear range of 1-20 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Report

Biotinylated Human ELISA

Immobilized Human Latent TGFB1, His Tagat 5 μg/mL (100 μL/well) can bind Human LRRC32, Fc Tag ith a linear range of 0.039-0.625 μg/mL (Routinely tested).

Protocol

活性(Bioactivity)-Cell based assay

Biotinylated Human CELL_BASE

Human Latent TGFB1, His Taginhibits the IL-4-dependent proliferation of TF-1 cells. The ED50 for this effect is 0.293-0.406 ng/mL (Routinely tested).

 

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