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Human LRRC32&TGF-beta 1 Heterotrimer protein, His Tag&Tag Free (MALS verified)

Human LRRC32&TGF-beta 1 Heterotrimer protein, His Tag&Tag Free (MALS verified)
别名: LRRC32 & TGF-beta 1,LRRC32&TGFB1
储存条件: -20℃ 
Human LRRC32&TGF-beta 1 Heterotrimer protein, His Tag&Tag Free (MALS verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-GA1-H52W9-100ug 100ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

 

Human LRRC32&TGF-beta 1 Heterotrimer protein, His Tag&Tag Free (MALS verified)

 

分子别名(Synonym)

LRRC32 & TGF-beta 1,LRRC32&TGFB1

表达区间及表达系统(Source)

Human LRRC32&TGFB1 Heterotrimer protein, His Tag&Tag Free  is expressed from human 293 cells. It contains AA His 20 - Asn 627 (LRRC32) & Leu 30 - Ser 390 (TGF-beta 1).

 

Request for sequence

蛋白结构(Molecular Characterization)

Human LRRC32&TGFB1 Heterotrimer protein, His Tag&Tag Free (the molar ratio of LRRC32 & TGF-beta 1 equals 1:2) is produced by co-expression of LRRC32 and TGF-beta 1, which has a calculated MW of 68.0 kDa (LRRC32), 28.5 (LAP) and 14.8 kDa (mature TGF-beta 1) respectively. LRRC32 is fused with a polyhistidine tag at the C-terminus and TGF-beta 1 contains no tag. The reducing (R) Heterotrimer protein migrates as 70 kDa (LRRC32), 38-45 kDa (LAP) and 14 kDa (mature TGF-beta 1) due to glycosylation respectively.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human LRRC32&TGFB1 Heterotrimer protein, His Tag&Tag Free (Cat. No. GA1-H52W9) SDS-PAGE gel

Human LRRC32&TGFB1 Heterotrimer protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%

The purity of Human LRRC32&TGFB1 Heterotrimer protein, His Tag&Tag Free is more than 85% and the molecular weight of this protein is around 150-184 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human LRRC32&TGFB1 Heterotrimer protein, His Tag&Tag Free at 1 μg/mL (100 μL/well) can bind Human TGFBR2, Fc Tag with a linear range of 0.3-5 ng/mL (QC tested).

 

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