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Mouse IL-17F Protein, His Tag

Mouse IL-17F Protein, His Tag
别名: IL-17F,Interleukin-17F,Cytokine ML-1
储存条件: -20℃ 
Mouse IL-17F Protein, His Tag
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-ILF-M5249-100ug 100ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

 

Mouse IL-17F Protein, His Tag

分子别名(Synonym)

IL-17F,Interleukin-17F,Cytokine ML-1

表达区间及表达系统(Source)

Mouse IL-17F, His Tag is expressed from human 293 cells. It contains AA Arg 29 - Ala 161.

Predicted N-terminus: His

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the N-terminus.

The protein has a calculated MW of 16.8 kDa. The protein migrates as 24-25 kDa and 27-28 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Mouse IL-17F, His Tag (Cat. No. ILF-M5249) SDS-PAGE gel

Mouse IL-17F, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

获取HPLC数据

活性(Bioactivity)-SPR

Biotinylated Human SPR

Human IL-17 RA, Fc Tag aptured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Mouse IL-17F, His Tag with an affinity constant of 2.99 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).

 

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