Biotinylated Human VEGF165, epitope tag free, ultra sensitivity (primary amine labeling)

Biotinylated Human VEGF165, epitope tag free, ultra sensitivity (primary amine labeling)

分子别名（Synonym）

RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF

表达区间及表达系统（Source）

MABSol® Biotinylated Human VEGF165, epitope tag free, primary amine labelings expressed from human  cells. It contains AA Ala 27 - Arg 191. It is the biotinylated form of ActiveMax® Human VEGF165, Tag Free (HPLC-verified) .

Predicted N-terminus: Ala 27

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries no "tag".

The protein has a calculated MW of 19.2 kDa. As a result of glycosylation, the protein migrates as 25-30 kDa (monomer) under reducing (R) condition, and 43-50 kDa under non-reducing (NR) condition (SDS-PAGE).

标记（Biotinylation）

The primary amines in the side chains of lysine residues and the N-terminus of the protein are conjugated with biotins using standard chemical labeling method. A standard biotin reagent (13.5 angstroms) is used in this product.

蛋白标记度（Biotin:Protein Ratio)

Passed as determined by the HABA assay / binding ELISA.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 24 months in lyophilized state;

2. -70°C for 24 months under sterile conditions after reconstitution.

 

电泳（SDS-PAGE）

Biotinylated Human VEGF165, epitope tag free, primary amine labeling on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

获取HPLC数据

 

活性（Bioactivity）-ELISA

Immobilized human VEGFR1/R2, Fc Tag at 2 μg/mL (100 μL/well) can bind Biotinylated Human VEGF165, epitope tag free, primary amine labeling with a linear range of 0.4-3 ng/mL (QC tested).

Protocol

Report

Immobilized Anti-VEGF MAb, Human IgG1 (Avastin) at 5 μg/mL (100 μL/well) can bind Biotinylated Human VEGF165, epitope tag free, primary amine labeling with a linear range of 0.2-2 ng/mL (Routinely tested).

Protocol

Immobilized Human Neuropilin-1, His Tagat 2 μg/mL (100 μL/well) can bind Biotinylated Human VEGF165, epitope tag free, primary amine labelingwith a linear range of 0.031-2 μg/mL (Routinely tested).

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