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| 别名: | FIGF,VEGFD | ||
|---|---|---|---|
| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
|---|---|
| 密度: | |
| 储存条件: | -20℃ |
Human VEGF-D Protein, His Tag
FIGF,VEGFD
Human VEGF-D, His Tag is expressed from human 293 cells . It contains AA Phe 93 - Ser 201 .
Predicted N-terminus: Phe 93
Request for sequence
）This protein carries a polyhistidine tag at the C-terminus.
The protein has a calculated MW of 13.4 kDa. The protein migrates as 19-22 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human VEGF-D, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
获取HPLC数据
Immobilized Human VEGF R3, Fc Tag at 5 μg/mL (100 μL/well) can bind Human VEGF-D, His Tag with a linear range of 0.08-0.6 μg/mL (QC tested).
Protocol
Human VEGF R3, Fc Tag captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human VEGF-D, His Tag with an affinity constant of 19.7 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Loaded Biotinylated Human VEGF R3, His,Avitag on SA Biosensor, can bind Human VEGF-D, His Tag with an affinity constant of 110 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Biotinylated Human VEGF R2, Avitag,His Tag on SA Biosensor, can bind Human VEGF-D, His Tag with an affinity constant of 482 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
