Human EGF R Protein, Fc Tag (MALS verified)

Human EGF R Protein, Fc Tag (MALS verified)

分子别名（Synonym）

EGFR,ERBB,ERBB1,HER1,PIG61,mENA

表达区间及表达系统（Source）

Human EGF R, Fc Tag  is expressed from human 293 cells. It contains AA Leu 25 - Ser 645 .

Predicted N-terminus: Leu 25

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 94.7 kDa. The protein migrates as 110-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>95% as determined by SDS-PAGE.

>90% as determined by SEC-MALS.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳（SDS-PAGE）

Human EGF R, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Human EGF R, Fc Tag is more than 90% and the molecular weight of this protein is around 230-260 kDa verified by SEC-MALS.

Report

 

活性（Bioactivity）-ELISA

Immobilized Human EGF, Mouse IgG2a Fc Tagat 2 μg/mL (100 μL/well) can bind Human EGF R, Fc Tag with a linear range of 0.3-5 ng/mL (QC tested).

Protocol

Immobilized Human EGF R, Fc Tag  at 1 μg/mL (100 μL/well) can bind Cetuximab with a linear range of 0.1-2 ng/mL (Routinely tested).

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