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Human HVEM / TNFRSF14 Protein, Mouse IgG2a Fc Tag, low endotoxin

Human HVEM / TNFRSF14 Protein, Mouse IgG2a Fc Tag, low endotoxin
别名: TNFRSF14,ATAR,HVEA,HVEM,LIGHTR,TR2,CD270
储存条件: -20℃ 
Human HVEM / TNFRSF14 Protein, Mouse IgG2a Fc Tag, low endotoxin
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-HVM-H5255-100ug 100ug 现货 3000.00 3000
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熔点:
密度:
储存条件: -20℃

Human HVEM / TNFRSF14 Protein, Mouse IgG2a Fc Tag, low endotoxin

分子别名(Synonym)

TNFRSF14,ATAR,HVEA,HVEM,LIGHTR,TR2,CD270

表达区间及表达系统(Source)

Human HVEM, Mouse IgG2a Fc Tag, low endotoxin  is expressed from human 293 cells . It contains AA Leu 39 - Val 202 .

Predicted N-terminus: Leu 39

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a mouse IgG2a Fc tag at the C-terminus.

The protein has a calculated MW of 44.4 kDa. The protein migrates as 55-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 0.1 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human HVEM, Mouse IgG2a Fc Tag, low endotoxin (Cat. No. HVM-H5255) SDS-PAGE gel

Human HVEM, Mouse IgG2a Fc Tag, low endotoxin on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human HVEM, Mouse IgG2a Fc Tag, low endotoxin  at 5 μg/mL (100 μL/well) can bind Human BTLA, Fc Tag with a linear range of 0.156-1.25 μg/mL (QC tested).

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