Anti-PP2C alpha antibody [3G1]

Anti-PP2C alpha antibody [3G1]

• 概述

• 产品描述The phosphorylation and dephosphorylation of proteins on serine and threonine residues is an essential means of regulating a broad range of cellular functions in eukaryotes, including division, homeostasis and apoptosis. A group of proteins that are intimately involved in this process are the serine/ threonine protein phosphatases. Protein phosphatase 2Ca (PP2Ca) has broad specificity. It dephosphorylates and negatively regulates the activities of MAP kinases and MAP kinase-kinases while also inhibiting the activation of p38 and JNK kinase cascades, induced by environmental stresses. PP2Ca also induces the expression of endogenous p53 and the p53-responsive gene p21, leading to cell cycle arrest and apoptosis. The PP2Ca protein, which contains an active site containing a dinuclear metal ion center, shows highest expression in epithelial cells, as well as in the digestive tract, lung, kidney, breast, prostate, endocrine glands and brain.
• 产品名称Anti-PP2C alpha antibody [3G1]
• 分子量46kDa
• 种属反应性Human,Mouse
• 验证应用WB,IP
• 抗体类型小鼠单抗
• 免疫原Full length PP2Cα of human origin.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm, Nucleus, Membrane
• 其它名称
  • Protein phosphatase 1A, Protein phosphatase 2C isoform alpha, Protein phosphatase IA

• 应用

  WB: 1:100-1:1,000
  IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)

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  Fig1: Western blot analysis of PP2Cα expression in non-transfected 293T (A), human PP2Cα transfected 293T (B) and HeLa (C) whole cell lysates.

   

  

  Fig2: Western blot analysis of PP2Cα expression in non-transfected 293T (A), human PP2Cα transfected 293T (B) and HeLa (C) whole cell lysates.

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