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Mouse IL-2 R beta&IL-2 R alpha&IL-2 R gamma Protein, Fc Tag&Fc Tag

Mouse IL-2 R beta&IL-2 R alpha&IL-2 R gamma Protein, Fc Tag&Fc Tag
别名: IL-2 R beta & IL-2 R alpha & IL-2 R gamma
储存条件: -20℃ 
Mouse IL-2 R beta&IL-2 R alpha&IL-2 R gamma Protein, Fc Tag&Fc Tag
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-ILG-M5253-5 100ug 现货 0.00
ZY-ILG-M5253-100ug 100ug 现货 3800.00 3800
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熔点:
密度:
储存条件: -20℃

 

Mouse IL-2 R beta&IL-2 R alpha&IL-2 R gamma Protein, Fc Tag&Fc Tag

分子别名(Synonym)

IL-2 R beta & IL-2 R alpha & IL-2 R gamma

表达区间及表达系统(Source)

Mouse IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tagis expressed from human 293 cells . It contains AA Ala 27 - Glu 240 & Glu 22 - Lys 236 & Trp 23 - Ala 263.

Predicted N-terminus: Ala 27 & Glu 22

蛋白结构(Molecular Characterization)

Mouse IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag is produced by co-expression of IL-2RB and IL-2RA and IL-2RG, has a calculated MW of 51.5 kDa (IL-2RB) and 79.4 kDa (IL-2RA&IL-2RG). Subunit IL-2RB is fused with a human IgG1 Fc tag at the C-terminus and subunit IL-2RA&IL-2RG is fused with a human IgG1 Fc tag at the C-terminus. The reducing (R) protein migrates as 75-90 kDa and 120-150 kDa respectively due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 . Normally Trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

After reconstitution, this product is stable after storage at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 电泳(SDS-PAGE)

Mouse IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag (Cat. No. ILG-M5253) SDS-PAGE gel

Mouse IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human IL-2, Tag Free  5 μg/mL (100 μL/well) can bind Mouse IL-2RB&IL-2RA&IL-2RG, Fc Tag&Fc Tag with a linear range of 0.05-0.313 μg/mL (QC tested).

 

 

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