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Human IL-23 R Protein, His Tag (MALS & SPR verified)

Human IL-23 R Protein, His Tag (MALS & SPR verified)
别名: IL-23 R,IL-23 Receptor
储存条件: -20℃ 
Human IL-23 R Protein, His Tag (MALS & SPR verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-ILR-H52H4-100ug 100ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

 

Human IL-23 R Protein, His Tag (MALS & SPR verified)

分子别名(Synonym)

IL-23 R,IL-23 Receptor

表达区间及表达系统(Source)

Human IL-23 R, His Tag is expressed from human 293 cells . It contains AA Gly 24 - Gly 355.

Predicted N-terminus: Gly 24

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 39.9 kDa. The protein migrates as 55-70 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human IL-23 R, His Tag (Cat. No. ILR-H52H4) SDS-PAGE gel

Human IL-23 R, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

The purity of Human IL-23 R, His Tag is more than 85% and the molecular weight of this protein is around 55-70 kDa verified by SEC-MALS.

Report

活性(Bioactivity)-SPR

Biotinylated Human SPR

Biotinylated Human IL-23A&IL-12B Heterodimer Protein, His,Avitag&Tag Free  captured on Biotin CAP - Series S sensor Chip can bind Human IL-23 R, His Tag (SPR verified)  affinity constant of 3.39 nM as determined in a SPR assay (Biacore 8K) (QC tested).

Protocol

 

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Biotinylated Human IL-23A&IL-12B Heterodimer Protein, His,Avitag&Tag Free on SA Biosensor, can bind Human IL-23 R, His Tag (SPR verified) with an affinity constant of 33.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 

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