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| 别名: | IL-23 alpha & IL-12 beta | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Human IL-23 alpha&IL-12 beta Heterodimer Protein, His Tag&Tag Free
IL-23 alpha & IL-12 beta
Human IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free is expressed from human 293 cells . It contains AA Arg 20 - Pro 189 (IL23A) & Ile 23 - Ser 328 (IL12B) .
Predicted N-terminus: His (IL23A) & Ile 23 (IL12B)
Request for sequence
Human IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free, produced by co-expression of IL-23 alpha and IL-12 beta, has a calculated MW of 19.5 kDa (IL-23 alpha) and 34.7 kDa (IL-12 beta). Subunit IL-23 alpha is fused with a polyhistidine tag at the N-terminus and subunit IL-12 beta contains no tag. The reducing (R) protein migrates as 20 kDa (IL-23 alpha) and 37-45 kDa (IL-12 beta) respectively due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized Human IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Freeat 2 μg/mL (100 μL/well) can bind Anti IL23A&IL12B P40 domain, Human IgG1 with a linear range of 0.05-3 ng/mL (QC tested).
Protocol
Immobilized Human IL-23 R, Fc Tag at 10 μg/mL (100 μL/well) can bind Human IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free with a linear range of 20-78 ng/mL (Routinely tested).
Protocol
Captured Human IL-23 R, Fc Tag on CM5 chip via anti-human IgG Fc antibodies surface can bind Human IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free with an affinity constant of 5.36 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Loaded Human IL-23 R, Fc Tag on Protein A Biosensor, can bind Human IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free with an affinity constant of 9.25 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Anti-IL23A & IL12B MAb (P40 domain) on Protein A Biosensor, can bind Human IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free with an affinity constant of 0.588 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human IL-12 R beta 1, Fc Tag on Protein A Biosensor, can bind Human IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free with an affinity constant of 1.97 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Human IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free stimulates secretion of IL-17 by mouse spleen cells. The ED50 for this effect is 0.1055-0.1635 ng/mL (Routinely tested).
Protocol
Cell based assay shows that the secretion of IL-17 induced by Human IL-23A&IL-12B Heterodimer Protein, His Tag&Tag Free is inhibited by increasing concentration of the anti-human IL-23 neutralizing antibody. The IC50 is between 0.67-1.50 ng/mL (Routinely tested).
