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Human IL-11 R alpha Protein, Fc Tag (MALS verified)

Human IL-11 R alpha Protein, Fc Tag (MALS verified)
别名: IL-11 R alpha,IL-11 RA,IL11RA,Interleukin-11 receptor subunit alpha,IL-11 receptor subunit alpha,IL-11R subunit alpha,IL
储存条件: -20℃ 
Human IL-11 R alpha Protein, Fc Tag (MALS verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-ILR-H5256-100ug 100ug 现货 3500.00 3500
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熔点:
密度:
储存条件: -20℃

 

Human IL-11 R alpha Protein, Fc Tag (MALS verified)

分子别名(Synonym)

IL-11 R alpha,IL-11 RA,IL11RA,Interleukin-11 receptor subunit alpha,IL-11 receptor subunit alpha,IL-11R subunit alpha,IL-11R-alpha

表达区间及表达系统(Source)

Human IL-11 R alpha, Fc Tag is expressed from human 293 cells . It contains AA Ser 24 - Ala 370

蛋白结构(Molecular Characterization)

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 64.3 kDa. The protein migrates as 70-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human IL-11 R alpha, Fc Tag (Cat. No. ILR-H5256) SDS-PAGE gel

Human IL-11 R alpha, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

The purity of Human IL-11 R alpha, Fc Tag  is more than 85% and the molecular weight of this protein is around 135-165 kDa verified by SEC-MALS.

Report

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Human IL-11, His Tag  at 5 μg/mL (100 μL/well) can bind Human IL-11 R alpha, Fc Tag with a linear range of 1-10 ng/mL (QC tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Human IL-11, His Tag  on HIS1K Biosensor, can bind Human IL-11 R alpha, Fc Tagwith an affinity constant of 0.177 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

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