Rhesus macaque HGF R / c-MET Protein,Fc Tag

Rhesus macaque HGF R / c-MET Protein,Fc Tag

分子别名（Synonym）

MET,AUTS9,HGFR,RCCP2,c-Met

表达区间及表达系统（Source）

Rhesus macaque HGF R,Fc Tagis expressed from human 293 cells. It contains AA Glu 25 - Asn 930 

Predicted N-terminus: Glu 25

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a human IgG1 Fc tag at the C-terminus. The mature form of HGF R is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. The protein has a calculated MW of 127.8 kDa (α chain 32.5 kDa and β chain 95.3 kDa). The protein migrates as ＞116 kDa (alpha & beta chain), 45 kDa (α chain) and 113-116 (β chain) kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳（SDS-PAGE）

Rhesus macaque HGF R,Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性（Bioactivity）-ELISA

Immobilized Human HGF, His Tagat 2 μg/mL (100 μL/well) can bind Rhesus macaque HGF R,Fc Tag with a linear range of 0.1-16 ng/mL (QC tested).

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