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| 别名: | CD258 antigen,CD258,HVEM-L,LIGHT,LTg,TNFSF14,TR2,HVEML | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Human LIGHT / TNFSF14 Protein, His Tag (active trimer) (MALS verified)
CD258 antigen,CD258,HVEM-L,LIGHT,LTg,TNFSF14,TR2,HVEML
Human LIGHT, His Tag (active trimer) (MALS verified)is expressed from human 293 cells. It contains AA Asp 74 - Val 240 trimer Design.
Predicted N-terminus: His
This protein carries a polyhistidine tag at the N-terminus.
The protein has a calculated MW of 58.3 kDa. The protein migrates as 55-65 kDa under non-reducing (NR) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human LIGHT, His Tag (active trimer) (MALS verified) on SDS-PAGE under non-reducing (NR) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.
The purity of Human LIGHT, His Tag (active trimer) (MALS verified)is more than 85% in HP-SEC, and the molecular weight of this protein is around 60-75 kDa verified by SEC-MALS.
Report
Immobilized Human HVEM, Fc Tagat 5 μg/mL (100 μL/well) can bind Human LIGHT, His Tag (active trimer) (MALS verified) with a linear range of 16-63 ng/mL (QC tested).
Protocol
Immobilized Human DcR3, Fc Tagat 5 μg/mL (100 μL/well) can bind Human LIGHT, His Tag (active trimer) (MALS verified) with a linear range of 8-63 ng/mL (Routinely tested).
Protocol
Human LIGHT, His Tag (active trimer) (MALS verified) induced cytotoxicity in HT-29 cells. The ED50 for this effect is 1.11-3.87 ng/mL (Routinely tested).
